2nd MPIZ-IBCH meeting

about ADIS:
- started in November 1995 (ADIS home page)
- ADIS-Gremium (dates of the "Gremiumstreffen")
- people working at ADIS
- instrumentation of ADIS
- informatics together with SUSAN (MPIZ network and computer support), and with ID-Pro GmbH (HTML and database programming)

DNA oligonucleotide ordering
- ordering via the OligoOrderDB (current program version c3.3)
- - local oligo E-commerce system with three suppliers, independent server, local database of all ordered oligos
- - see the "frequently asked questions"-page (OligoOrderDB-FAQ)
- - how should I type my "Kostenstelle"? See the "Kostenstellenliste"
- - number of orders processed per week (graphic)
- group-specific access to the MPIZ oligo database
- - store information on your existing oligos, or on oligos from other sources
- - add comments to existing entries ("edit" function - add comments to older DB entries)
- - use the statistics information to see how much money was spent
- how the "Einkauf" uses the generated data
- three suppliers: see the price lists of MWG, LifeTechnologies/GibcoBRL, and metabion)
- export option of a user-specific list of oligos for "Material" in diploma and PhD theses (tab-delimited text file)
- required: maintenance (if you report bugs we will try to fix them ....)

DNA sequencing
- general info and recommendations
- our collection of "standard" primers which you do NOT need to supply
- BigDye-Terminator chemistry
- number of reactions (per month, only those charged - sorry, so far only data from 1999)
- for M & M: if you use sequencing data in publications
- collection of a few "problems & (a few) solutions" in automated fluorescent DNA sequencing
- small sequencing projects (fragments significantly larger than 1.4 kbp - see the sequencing info; software: Sequencher)
- data management with a LIMS system: SeqOrder1.3 (first version completed 08/1999)
- - see the "frequently asked questions"-page (SeqOrder-FAQ)
- - user access page
- - ADIS operator access page (how are the data processed?)
- - - WWW-based generation of sequencing requests (database of all requests)
- - - correct username, complete KSt info (all from UserDB, the access control system)
- - - new users will be added upon request
- - - automatic generation of data file names (01-3456-username-temp-prim.Seq)
- - - computer-aided generation of "sample sheets" to program the sequencers
- - - automatic listing of "requests in queue", request tracking (usually user-specific - not in this demo)
- - - computer-aided generation of the monthly "Umbuchungsbelege"
- - automatic data delivery and access to old data as well as the trace files
- - - data delivery to users, directed by file names (01-3456-username-temp-prim.Seq - script by Stephan Webanck)
- - - automatic data backup, directed by file names (/home/adis/adis/backup/0/0/8/8/008812)
- - - user access to data files (sequence text and "trace" files) with getadisdata
- planned for the future:
- - better base caller which includes accuracy values (UNIX-based)
- - improved automated blast searches (automatic vector trimming, WebBlast, data access via IntraNet)
- - paper-free data delivery (delivery sheet via IntraNet, Java-based trace file viewer from your Web Browser)

DNA fragment analysis (GeneScan)
- support for mapping experiments
- fluorescent differential display
- SSCP analysis on 310 and on the 3700 (SNP detection)

Colony picking and spotting
- service for people interrested in picking and spotting
- joined efford to generate A. thaliana cDNA filters (PCR fragments from ESTs, example picture from controls)
- establishment of Glas chip technology (Sirak Kifle)

Other stuff
- support for mapping using A. thaliana RILs (DNA of Lister and Dean lines)