Insertion mutagenesis populations

Listing of insertion mutagenesis populations theoretically available for reverse genetics in A. thaliana (as far as we know; at MPIZ and arround the world).

T-DNA populations

population accession features (1)
insert's/line
features (2) # of lines FSTs in database search by seed availability MTA alternative
screening
reference
GABI-Kat
(to search: SimpleSearch)
Col
1.5
activation tag
15.000
20000 (genome hits)
4500 (gene hits)
sequence,
accession
MPIZ (B. Weisshaar)
June 2002
yes
no
?
ZIGIA T-DNA's
(FSTs in GenBank/EMBL)
Col 1.5 activation tag
8.000
211
(genome hits)
sequence,
accession
MPIZ
contact: B. Reiss
yes no ?

Versailles

Ws
1.5
?
50.000
3.803
sequence
01.10.2001
?
?
Balzergue et al.
Ecker (SIGnAL)
Col
?
?
150.000
11.745
sequence,
accession
personal arrangement
contact: J. Ecker
?
?
?
Szabados (Vitality)
?
?
promoter trap
?
1.200
accession
personal arrangement
contact: L. Szabados
yes
?
?
Syngenta (GARLIC)
Col
?
?
107.000
21.000
(gene hits)
sequence
personal arrangement
contact: S. Goff
yes
no
?
Koncz
?
?
2-3 ins.
39.700
no
-
personal arrangement
contact: C. Koncz
n.a.
PCR
?
Wisconsin (AFGC)
Ws
?
activation tag
?
60.480
72.960
?
-
ABRC
n.a.
PCR (2x 324.- $)
?

Transposon populations

population ecotype features (1)
insertions/line
features (2) # of lines FSTs in database search by seed availability MTA alternative screening reference
ZIGIA (Wisman/Dekker)
Col
~8
En-1
10.000
no
-
MPIZ,
(Koen Dekker)
yes
PCR,
hybridisation filters
Wisman et al.
SLAT (J. Jones)
(search:
NASC)
Col

1.2

dSpm
60.000
1.200
sequence,
accession
NASC,
Yvette Reader
yes
PCR,
hybridisation filters
Tissier et al.
IMA (Sundaresan)
Ler

1.2

Ds
gene/enhancer trap
?
1.000
accession
ABRC
yes
no
?
CSHL (R. Martienssen)
Ler
?
Ds
gene/enhancer trap
?
10.000
sequence,
accession
?
?
no
?
ITS (A. Pereira)
Ler
~40
En-1/Spm
20.000
?
-
personal arrangement
contact: A. Pereira
?
no
?
  • There are some useful reviews on knockouts:
    • Using gene knockouts to investigate plant metabolism.Thorneycroft D, Sherson SM and Smith SM: Journal of Experimental Botany 2001: 52, 1593-1601 (abstract).
    • Arabidopsis gene knockout: phenotypes wanted. Bouche N and Bouchez D: COPB 2001: 4, 111-117 (abstract)
  • If you know a population which is not listed here, let me know! Please report broken links, typos and other problems as well! BW
  • The "?" does not mean that this information is not existing. It only means that we have not yet found it - if you know the "real value" which should replace any of the "?" please let me know. BW.